High-Purity Tris Acetate 6850-28-8 Biochemical Reagent Ingredients Factory Direct Sales

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Product Description

Basic Information

Model NO.TAE Buffer
EINECS229-939-6
ClassificationBiochemical Reagents
GradeAR
ContentPurity Above 99%
StorageRoom Temp
Molecular Weight181.19
Melting Point120-121°C
Specification500g/bottle

Product Description

Packaging Specification: Plastic bottles, 500g/bottle.

Basic Information Table

Product NameTris(hydroxymethyl)aminomethane acetate salt
SynonymsTRIS(HYDROXYMETHYL) AMINOMETHANE ACETATE extrapure AR;TRIZMA ACETATE;TAE;TAE BUFFER
CAS6850-28-8
MFC6H15NO5
MW181.19
EINECS229-939-6
Product CategoriesBiochemistry;Good's Buffers;Buffer
Tris Acetate

Introduction to TAE buffer

TAE buffer is widely used as a nucleic acid electrophoresis buffer, mainly for DNA agarose gel electrophoresis in biology. When performing electrophoresis of segments larger than 13 kb, using TAE buffer achieves a better separation effect. It is also easy to use the TAE buffer system for electrophoresis when recovering DNA fragments. The disadvantage of TAE is its small buffer capacity, which cannot be used for long-term electrophoresis (such as overnight).

TAE Buffer Application
TAE Buffer Usage

Product Advantages

Purity ≥ 99%, good water solubility, stable process, and can ensure that the product appearance is pure white crystal powder.

50× TAE Buffer Configuration Method

1. Weigh 242g of Tris and 37.2g of Na2EDTA.2H2O in a 1L beaker;
2. Add approximately 800ml of deionized water to the beaker and stir thoroughly until uniform;
3. Add 57.1ml of glacial acetic acid and dissolve thoroughly;
4. Add deionized water to a constant volume of 1L and store at room temperature.

TAE Buffer Configuration
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FAQ

What is the primary use of TAE buffer?
TAE buffer is mainly used for DNA agarose gel electrophoresis in biological research, especially effective for segments larger than 13 kb and recovering DNA fragments.
What is the purity level of this TAE buffer?
This product features a high purity level of above 99%, ensuring stable performance for laboratory use.
How should the TAE buffer be stored?
It should be stored at room temperature to maintain its properties.
Can this product be used for long-term electrophoresis?
No, TAE has a small buffer capacity, so it is not recommended for long-term electrophoresis, such as overnight runs.
What are the key advantages of this TAE buffer?
Key advantages include high purity (≥99%), excellent water solubility, a stable production process, and a consistent, pure white crystalline powder appearance.

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