Product Description
Packaging Specification: Plastic bottles, 500g/bottle
| Product Name | Tris(hydroxymethyl)aminomethane acetate salt |
| Synonyms | TRIS(HYDROXYMETHYL) AMINOMETHANE ACETATE extrapure AR; TRIZMA ACETATE; TAE; TAE BUFFER |
| CAS | 6850-28-8 |
| Molecular Formula | C6H15NO5 |
| Molecular Weight | 181.19 |
| Product Categories | Biochemistry; Good's Buffers; Buffer |
Introduction to TAE Buffer
TAE buffer is a widely used nucleic acid electrophoresis buffer, primarily utilized for DNA agarose gel electrophoresis in biological research. When separating DNA segments larger than 13 kb, the TAE buffer system provides superior separation effects. It is also the preferred system for recovering DNA fragments after electrophoresis.
Note: Due to its relatively small buffer capacity, TAE is not recommended for long-term electrophoresis (such as overnight runs).
Product Advantages
✔
High Purity: ≥ 99%, ensuring reliable experimental results.
✔
Excellent Solubility: Good water solubility for quick preparation.
✔
Stable Process: Pure white crystal powder appearance with consistent quality.
50× TAE Buffer Configuration Method
1.
Weigh 242g of Tris and 37.2g of Na2EDTA.2H2O into a 1L beaker.
2.
Add approximately 800ml of deionized water and stir thoroughly until uniform.
3.
Add 57.1ml of glacial acetic acid and dissolve completely.
4.
Adjust the final volume to 1L with deionized water and store at room temperature.
Frequently Asked Questions
What is the main application of TAE Buffer?
It is primarily used for nucleic acid electrophoresis, specifically DNA agarose gel electrophoresis, and is ideal for separating DNA fragments larger than 13 kb.
What is the purity level of your Trizma Acetate?
The product features high purity of 99% or above, provided as a pure white crystal powder.
Can TAE Buffer be used for overnight electrophoresis?
No, it is not recommended for long-term or overnight electrophoresis because of its relatively small buffer capacity.
How should the TAE buffer powder be stored?
The powder should be stored at room temperature in a dry place to maintain its stability.
What is the standard packaging size?
The standard packaging is 500g per plastic bottle, but OEM packing is also available.
Why choose TAE over other buffer systems?
TAE is excellent for recovery of DNA fragments and provides better separation for large DNA segments (greater than 13 kb).